【摘要】：目的对三种不同的检测BALB/c小鼠是否受孕的方法进行比较研究。方法只检测阴栓(方法一)、检测阴栓结合称量体质量(方法二)、只称量体质量(方法三),用3种方法检测同一组雌鼠的受孕情况并比较各方法的检测能力。结果方法一检测小鼠受孕的灵敏度为0.71、特异度为0.47,方法二灵敏度为0.71、特异度为0.94,方法三灵敏度为0.96、特异度为0.94。结论在对受孕(gestation day,GD)10天及后期孕鼠的检测方法中,称量体质量较其它方法更为可靠和实用。
【作者单位】： 山西医科大学第一医院口腔科;
【关键词】： BALB/c小鼠 受孕 检测方法
【基金】：山西省自然科学基金2013011056-5
【分类号】：S865.13
【正文快照】：
在应用小鼠的实验研究中,经常需要在特定孕期以一定方式干预孕鼠,所以如何准确检测小鼠是否受孕成为进行此类实验常常面临的问题。一般采用阴栓检测法、阴道精子检测法来确定小鼠是否受孕,但事实上该类方法只能确定小鼠进行交配,并不能真实反映实际受孕情况[1]。另有学者采用
Abstract： Abstract:Objective To compare and study three inspecting methods which test whether the mice were pregnant or not． Method Check and study the fertilization of the same mice group with three methods． The first method was to use vaginal suppository to identify the mice were pregnant or not; the second method was to use vaginal suppository and check mice’s weight; the third method was to check mice’s weight． Ｒesult The first method sees the sensitivity of the mice’s pregnancy was 0. 71 and the specificity was 0. 47． According to the second method，the sensitivity was 0. 71 and the specificity was 0. 96． And third method shows that the sensitivity was 0. 96 and the specificity was 0. 94． Conclusion For the methods detecting mouse gestation GD 10 and late state，observing change of weight is more reliable and practical than other methods in the process of inspecting pregnant mouse in the GD 10 period and late stage．
Keywords： BALB / c mice pregnancy inspecting method
【作者单位】： 山西医科大学第一医院口腔科;
【关键词】： BALB/c小鼠 受孕 检测方法
【基金】：山西省自然科学基金2013011056-5
【分类号】：S865.13
【正文快照】：
在应用小鼠的实验研究中,经常需要在特定孕期以一定方式干预孕鼠,所以如何准确检测小鼠是否受孕成为进行此类实验常常面临的问题。一般采用阴栓检测法、阴道精子检测法来确定小鼠是否受孕,但事实上该类方法只能确定小鼠进行交配,并不能真实反映实际受孕情况[1]。另有学者采用
Abstract： Abstract:Objective To compare and study three inspecting methods which test whether the mice were pregnant or not． Method Check and study the fertilization of the same mice group with three methods． The first method was to use vaginal suppository to identify the mice were pregnant or not; the second method was to use vaginal suppository and check mice’s weight; the third method was to check mice’s weight． Ｒesult The first method sees the sensitivity of the mice’s pregnancy was 0. 71 and the specificity was 0. 47． According to the second method，the sensitivity was 0. 71 and the specificity was 0. 96． And third method shows that the sensitivity was 0. 96 and the specificity was 0. 94． Conclusion For the methods detecting mouse gestation GD 10 and late state，observing change of weight is more reliable and practical than other methods in the process of inspecting pregnant mouse in the GD 10 period and late stage．
Keywords： BALB / c mice pregnancy inspecting method
